Hey, I’m Back With Another Masterpost. I Decided To Include Some Of My Tips.

Dose Dependent effects of Dopamine mnemonic

Simplified version of Dopamine’s dose-dependent MAJOR effects:

low doses - activates D1 receptors (Gs) - vasodilates renal blood vessels*

med.doses - activates B1 receptors (Gs) - increases heart rate/contractility etc..

high doses - activates A1 (Gq) - vasoconstricts,

*increased renal perfusion, GFR, also vasodilates mesenteric and coronary vessels 

source: http://reference.medscape.com/drug/intropin-dopamine-342435

-28/11/17-

Had cramps so spent the day writing up microbiology notes whilst hunched over my hot water bottle. The lecture on vaccine design is 7 pages long and now I can hand cramp to my list of ailments…

Pneumonia & Meningitis Timelines

volutin granules are an intracytoplasmic storage form of complexed inorganic polyphosphate, the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler’s medium….look like chines letters…as given below

Hope it helps @shreeparn  :)

Antimicrobial Agents - Inhibition of DNA and Protein Synthesis

Bacterial chromosome replication

DNA replication

Bacterial Topoisomerases 

maintain DNA in appropriate state of supercoiling 

cut and reseal DNA

DNA gyrase (topoisomerase II) introduces negative supercoils 

Topoisomerase IV decatenates circular chromosomes 

these are the targets of the quinolone antibacterial agents 

Quinolones

bind to bacterial DNA gyrase and topoisomerase IV after DNA strand breakage 

prevent resealing of DNA 

disrupt DNA replication and repair 

bactericidal (kill bacteria)

Fluoroquinolone is particularly useful against

Gram +ves: Staphylococcus aureus, streptococci 

Gram -ves: Enterobacteriacea; Pseudomonas aeruginosa 

Anaerobes: e.g. Bacteroides fragilis 

many applications e.g. UTIs, prostatitis, gastroenteritis, STIs 

Adverse effects

Relatively well tolerated

GI upset in ~ 5% of patients 

allergic reactions (rash, photosensitivity) in 1 - 2% of patients 

Inhibition of Bacterial Protein Synthesis 

Macrolides 

in 1952: Erythromycin was isolated as the first macrolide (Streptomyces erythreus) 

Newer macrolides: clarithromycin, azithromycin 

Structurally they consist of a lactone ring (14- to 16-membered) + two attached deoxy sugars 

Mode of action 

bind reversibly to bacterial 50S ribosomal subunit 

causes growing peptide chain to dissociate from ribosome → inhibiting protein synthesis 

bacteriostatic (stops reproduction)

Macrolides’ spectrum of activity

good antistaphylococcal and antistreptococcal activity 

treatment of respiratory & soft tissue infections and sensitive intracellular pathogens • e.g. Chlamydia, Legionella 

Adverse effects

Generally well tolerated

nausea 

vomiting 

diarrhoea 

rash 

Aminoglycosides

large family of antibiotics produced by various species of Streptomyces (“mycin”) and Micromonospora (“micin”) 

include: streptomycin, neomycin, kanamycin, gentamicins, tobramycin 

Structure = linked ring system composed of aminosugars and an aminosubstituted cyclic polyalcohol 

Mode of action of aminoglycosides

Bind irreversibly to 30S ribosomal subunit 

disrupt elongation of nascent peptide chain 

translational inaccuracy → defective proteins 

bactericidal 

Spectrum of activity 

broad spectrum; mainly aerobic G-ve bacilli (e.g. P. aeruginosa) 

used to treat serious nosocomial infections (hospital acquired infections)

First TB antibiotic

Used for cystic fibrosis 

Adverse effects

all aminoglycosides have low Therapeutic Index (only a small amount needed to become toxic)

renal damage, ototoxicity, loss of balance, nausea 

Dr Warhol’s Periodic Table of Microbes

56. Ba. Bacillus

There are more than 300 species of Bacillus, which is a whole whopping load of microbes. Just to wrap your head around that number, if you talked about each one for 1 minute you’d be talking nonstop for 5 hours! Take that TedTalks!

Starting with the basics, these organisms found all over the world, predominantly in soil but microbes go where they please, so they have been found in undersea hydrothermal vents as well as in the stratosphere. They are rod-shaped and form spores.

Just to list a few of the most noteworthy and awesome Bacillus species:

Abyssalis: found more than a mile and a half down at the bottom of the South China Sea.

Anthracis: causative agent of Anthrax, the disease, not the band; death, disease, toxins, yahoo!

Azotofixans: fixes nitrogen.

Canaveralius: StarFleet Academy space bacteria living on the walls of the Kennedy Space Center!

Cereus: you get to play with this in General Microbiology, a pathogen causing foodborne illness.

Decolorationis: for you art history majors, isolated from decaying parts of a mural in the Roman necropolis in Carmona, Spain.

Megaterium: it can consume cave paintings.

Stratosphericus: found in high concentrations orbiting the Earth with satellites around 6 miles up!

Subtilis: the grass bacillus; used for industrial enzyme secretion.

Thuringiensis: absolutely famous for producing the BT toxin used as a natural insecticide.

Bacillus cells are Gram positive rods that measures about 1 micron wide by 4 to 10 microns long, but with more than 300 species you will see a range of sizes.

Everyone needs their own Periodic Table of Microbes from https://www.etsy.com/no-en/shop/WarholScience.

Copyright 2016 Warhol.

Positives are violet in color and negatives are red or pink on gram stain! My untidy handwritten notes here.

Who wants a box of chocolates when you can have a petri dish of bacteria?